on-rate vs. off-rate

Morten Lindow lindow at mdb.ku.dk
Fri Feb 4 10:33:37 EST 2000


 Furthermore, they say that when antibody affinities become
> higher this is mostly due to a lower off-rate, with the on-rate being
> less important. I kind of have problems to completely understand this.
> Is there anybody who could explain this to me, or has anyone a reference
> in which this is clearly explained?
I don't know if this is what you are looking for, but:
In case you don't already know, the dissociation constant is closely related
to the kinetic rate constants:

Kd= koff / kon

A thourough explanation of this can be found in any textbook on physical
chemistry.

Regards,
Morten






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