More general questions about FACS

Kevin Ault kevin-ault at
Fri Feb 11 09:00:12 EST 2000

I have a rather basic question about FACS as well.  If one is looking
for a response to a certain antigen, what is the best source of
antigen presenting cells?   Autologous irradiated macrophages?  Mixed
peripheral mononuclear cell cultures?   Something else?   I am mainly
interested in humans.  Please post replies to the group. -   Kevin
Ault MD University of Iowa  (kevin-ault at 

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