immunohistochemistry of chemokines

lb lbaloulNOlbSPAM at
Fri Feb 18 17:29:59 EST 2000

I have a problem with  detection of MCP-1 by
immunohistochemistry on brain section.I used PFA for
fixation and I have not obtained any staining but when I
used acetone I obtained a staining for MCP-1 and when
compared to the section without the primary AB I have not
the staining. is that a specific staining(because I not
block the FC receptors)? and what is the differences in the
utilization of PFA or acetone for the fixation?

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