How to remove bovine immunoglobulins?

Phillip E. Schwartz schwartz at bioreagents.com
Fri Feb 25 18:57:50 EST 2000


We offer a product, recombinant protein L agarose, which can completely
discriminate between bovine and mouse IgG. Please visit our website or call
for additional product information.

http://www.bioreagents.com/
http://www.bioreagents.com/proteinl.html

Best regards,
Phil



nan at inflam.lu.se wrote:

> Hello friends,
>
> I am using 5-10% of Fetal bovine serum containing medium to culture my B-
> cell hybridomas. While purifying the culture supernatants using Protein G
> column (Gama bind plus), I am facing the problem of bovine
> immunoglobulins copurifying with my monoclonal antibodies. How to remove
> the bovine immunoglobulins contamination from my purified monoclonal
> antibodies? Is there any company marketing affigel columns with anti-
> bovine immunoglobulins? or is there any alternative methods available?
> Now I have started using either protein A passed FBS or Ultra low bovine
> immunoglobulin containing sera for the culture medium to circumvent this
> problem. Any help in this regard will be useful. Thanks in advance for
> your suggestions.
>
> regards,
> K.S.Nandakumar
>
> Sent via Deja.com http://www.deja.com/
> Before you buy.
-------------- next part --------------
A non-text attachment was scrubbed...
Name: schwartz.vcf
Type: text/x-vcard
Size: 354 bytes
Desc: Card for Phillip E. Schwartz
Url : http://iubio.bio.indiana.edu/bionet/mm/immuno/attachments/20000225/99174ae0/schwartz.bin


More information about the Immuno mailing list