problem with an ELISA

Guy Hermans guy.hermans at stanford.edu
Thu Jan 6 01:26:32 EST 2000


Christiane Devaux wrote:

> What did I have to change to clean my results ? I read a paper about
> dilution of plasma but I don't think it's a good idear. Is there another
> solution ???
A typical problem is the presence of rheumatoid factor in sera:
antibodies that bind the Fc of other antibodies. This crosslinks your
detecting antibody with the capture antibody, giving "Background". The
best way to avoid this is to use a F(ab)2 fragment for either capture
or detection. Yes, it's more expensive (and harder to find
commercially), but well worth it in terms of data quality.

Good luck,

Guy

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Guy Hermans, PhD

Dept. of Neurology and Neurological Sciences
Beckman Institute
Stanford University, CA 94305-5429

Tel. (650) 723-6757
Fax. (650) 725-0627
e-mail guy.hermans at stanford.edu
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"Duct tape's mystical: it's got a light side, a dark side and
it's the force that hold the universe together"        -Anon.
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