Vaccinia Detection

[Nadeem Sheikh]

Any suggestions as to how I can detect/quantify vaccinia infection ?

Background : I employ recombinant vaccinia as vectors, infecting autologous
B-LCL lines with them.  These infected cells are then used as antigen
presenters to purified  PBMC.  I cannot look for the genes/gene products
that the vaccinia carries as reagents for these are not available.  I would
ideally like to be able to use flow cytometry to be able to gauge the
efficacy (and even amount) of infection - just due to the fast turn around
time for facs .

Any suggestions to techniques/antibodies available.  Any info would be
greatly appreciated.  Thanks !


Nadeem Sheikh,
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RPRC                                                     Department of
Pharmaceutics
University of Washington                         University of Washington
Box 357331                                            Box 357610
Seattle, WA 98195                                  Seattle, WA 98195

voice : +1 206 221 2356
    fax : +1 206 956 0573
e-mail : nadeem at bart.rprc.washington.edu
http://www.rprc.washington.edu/