I used to do that by extracting the bands and perform a dot blot.
Subcloning and sequencing would be the other solution...
On Sun, 23 Jan 2000 15:47:26 +0800, "Chong Wai Yin( Zhang Weixian)"
<wychong at singnet.com.sg> wrote:
>Hi, is there anyway to know if the extra bands after PCR is due to
>unspecific binding or due to fragmented DNA-of-interest. (eg. gene of
>interest is fragmented and after PCR, the fragment is amplified.). Thanks.
>Chong Wai Yin, Kelvin
>-- For Nature, heartless, witless Nature
> Will neither know nor care.
> And from my pillow, looking forth by light
> Of moon or favouring stars, I could behold
> The antechapel where the statue stood
> Of Newton with his prism and silent face,
> The marble index of a mind for ever
> Voyaging through strange seas of Thought, alone. --