PCR

Chris Larosa clarosa at biocomp.unl.edu
Mon Jan 24 19:35:40 EST 2000


Southern blot analysis would tell you, subcloning the fragments and sequencing
would tell you

"Chong Wai Yin( Zhang Weixian)" wrote:

> Hi, is there anyway to know if the extra bands after PCR is due to
> unspecific binding or due to fragmented DNA-of-interest. (eg. gene of
> interest is fragmented and after PCR, the fragment is amplified.). Thanks.
>
> --
> Chong Wai Yin, Kelvin
> Zhang Weixian
> -- For Nature, heartless, witless Nature
>    Will neither know nor care.
>    And from my pillow, looking forth by light
>    Of moon or favouring stars, I could behold
>    The antechapel where the statue stood
>    Of Newton with his prism and silent face,
>    The marble index of a mind for ever
>    Voyaging through strange seas of Thought, alone. --





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