>Hi, is there anyway to know if the extra bands after PCR is due to
>unspecific binding or due to fragmented DNA-of-interest. (eg. gene of
>interest is fragmented and after PCR, the fragment is amplified.). Thanks.
>
How can a fragmented DNA be amplified? It had to be fragmented and
re-ligated in order to give a PCR product. This should be a very rare case
- and if the specific template is present in - lets say a few ten thousand
copies (if you use low amounts of DNA) - the rare religated fragment
should not be amplified enough to give a band.
Or am I mistaken??
Gregor
--
Gregor Bucher, Zoologisches Institut der LMU München
0049/89/5902/444
st2153 at zi.biologie.uni-muenchen.de
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