In article <s48h4.220$M47.4708 at news1-hme0>,
"Hiromi Tagoh" <htagoh at hotmail.com> wrote:
>> "Alberto Bergamini" <bergamini at Med.uniroma2.it> wrote in message
> news:38849812.BB30C53C at Med.uniroma2.it...> > Could anyone tell me which of the following HCV peptides are able to
> > induce cytokine production in in vitro cultured PBMC from patients
with
> > chronic hepatitis C? Also, does anyone know how long the stimulation
> > period should be , using cytofluorimetric analysis as the detection
> > method?
> > Peptides:
> > 1) core, aa. 19-25
> > 2) core, aa. 107-174
> > 3) E2, aa.484-499
> > 4) NS4, aa. 1658-1863.
> > Thank you in advance
> > Alberto Bergamini, M.D.
> > Dept of Public Health
> > University of Rome
> >
> > ---
>> You want to detect cytokine production by using cytofluorimetric
assay? It
> seems to be difficult.
>> yes, it seems diffucult to me too!!! but what about "antigen-specific
cytokine response to hepatitis C virus core epitopes in HIV/hepatitis C
virus-coinfected patients" by Woitas RP, Rockstroh JK et al. pub. in
AIDS, 13(11):1313-22 1999 jul 30, have you read it?
I would like to hear your critics about it.
Alberto Bergamini
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