Immunoprecipitation question

Neal Robert Melvin nrmelvin at ucalgary.ca
Mon Jan 31 02:16:19 EST 2000

When I've pelleted my beads during washing steps, they (obviously) all
clump together in one big ball... is it advisable to break this clump up
by vortexing to disperse the beads?? I'm assuming this will allow more
efficient washings, but I'm worried that vortexing might knock my antigen
off. Any suggestions??

Please email me directly, as  I rarely have access to newsgroups.

Thanks in advance,


"Everyone tells me I'm lucky,
I've got my whole life to live yet,
I can't say they're wrong, 
but the days seem so long
Living inside of my head
Maybe I'll get some relief now,
Now that your things are all gone
I won't sit here staring at nothing all night
Bleary-eyed, greeting the dawn"

'Falling Down Blue'

Neal Melvin
University of Calgary - Faculty of Medicine
Department of Neuroscience - Neuroscience Research Group (NRG)
Health Sciences Centre
3330 Hospital Drive, NW
Calgary, Alberta, Canada
T2N 4N1

e-mail: nrmelvin at ucalgary.ca
Phone: (403) 220-4490 (lab)
       (403) 220-7035 (office)
       (403) 283-8731 (fax)

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