isotype switch in established B cell hybridoma

nan at inflam.lu.se nan at inflam.lu.se
Mon Jul 17 07:02:08 EST 2000


Dear friends,

We are interested in isolating the isotype switch variants of
an established, stable B-cell hybridoma. Now, the hybridoma
secretes IgG1 antibodies. I would like to use gamma interferon
with the hybridoma to get the IgG2a antibodies.
Is there any established protocol available or possible
references? Any lead will be highly useful.

With regards,
K.S.Nandakumar


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