nitrocellulose blotted antigen

Mark Haynes Mark.Haynes at Mail.TJU.Edu
Mon Jul 17 17:39:49 EST 2000


I remember a paper back in the 80"s,  I think it appeared in immunology,
that dealt with immunizing with blotted antigens when attempting to make
monoclonals

jarrat jordan wrote:

> Can anyone reccomend me a protocol on using blotted protein as an
> antigen.
> I am curious on how much protein to blot, whether or not to block, how
> to send it off etc.
>
> thanks in advance,
> Jarrat






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