Paraformaldehyde

Roshni Mitra roshni at ccmb.ap.nic.in
Tue Jul 18 00:40:43 EST 2000


Hi! I have used formaldehyde for fixing cells... it does not disrupt the
memebrane as far as I know, it just causes cross-linking of the outer
membrane components and hence, fixes it...so no membrane secretion,
endocytosis etc can take place but the cell structure as such remans
intact.

On Mon, 17 Jul 2000, Christiane Meyer wrote:

> Hi there,
> 
> we use paraformaldehyd to fix our cells for FACS analysis. I was
> wondering what effects PFA has on cell, especially on the cellmembrane.
> I searched  the web, but couldn´t find any info on the effects of
> chemicals. Does anyone have a link that would help?
> 
> 
> Thanks
> Christiane
> 
> 
> 


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