MHC @ trypsin

David R. Johnson david.johnson at yale.edu
Wed Jul 19 08:03:38 EST 2000


Alec-

MHC class I molecules on the surface of cultured cells are RESISTANT to
trypsin treatments used to suspend the cells.  I do this routinely to
study expression by FACS.  Purified MHC class I molecules are sensitive
to relatively large amounts of trypsin.  Sorry I cannot cite a reference
off the top of my head, but it has been shown by different groups.

I hope this helps.

-Dave

--
David R. Johnson, Ph.D.
Research Scientist, Department of Pathology
454 BCMM, 295 Congress Avenue
Yale Medical School, New Haven, CT 06510 USA
Tel.: 203/737-2298, Fax: 203/737-2293
http://johnsond3.med.yale.edu







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