MHC @ trypsin

Dr Alec Redwood aredwood at cyllene.uwa.edu.au
Thu Jul 20 00:44:15 EST 2000


Thanks for that, it was what I remembered

"David R. Johnson" wrote:

> Alec-
>
> MHC class I molecules on the surface of cultured cells are RESISTANT to
> trypsin treatments used to suspend the cells.  I do this routinely to
> study expression by FACS.  Purified MHC class I molecules are sensitive
> to relatively large amounts of trypsin.  Sorry I cannot cite a reference
> off the top of my head, but it has been shown by different groups.
>
> I hope this helps.
>
> -Dave
>
> --
> David R. Johnson, Ph.D.
> Research Scientist, Department of Pathology
> 454 BCMM, 295 Congress Avenue
> Yale Medical School, New Haven, CT 06510 USA
> Tel.: 203/737-2298, Fax: 203/737-2293
> http://johnsond3.med.yale.edu






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