Aggregation of IgG during purification

Tarmo Humppi tarmo.humppi at pvtt.mil.fi
Wed Jun 28 02:25:55 EST 2000


We have tried to purify polyclonal rabbit antibodies from serum using
affinity column that has the peptide antigen attached to it. The
problem is that the eluted IgG aggregates immediately after elution. We
have used Tris buffer pH 7.5 as starting buffer and glysine pH 3.0 for
elution. Is there a way to get the aggregated IgG back to solution? We
have tried increasing ionic strenght but it hasn´t helped. All tips are
appreciated!

--
Tarmo


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