Monensin v BFA

Andrew Hall andrew.hall at virgin.net
Thu Mar 16 13:53:38 EST 2000


I have been debating whether to use BFA or monensin to block golgi secretion
of cytokine before intracellular flow cytometery staining of human PBMC
cultures. As far as I am aware monensin is probably the best candidate since
I have read that BFA can affect the expression of some CD markers (CD69)
although it apparently has greater toxicity. However I have been unable to
find a protocol or paper with the correct concentration and volume of
monensin to add to a given number of cells. Any suggestions to these queries
would be appreciated.

--
Andrew Hall
University of Aberdeen
andrew.hall at virgin.net







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