Gel filtration

Onyryc onyryc at free.fr
Wed Mar 22 19:12:46 EST 2000


How to interpreter these results:

Sephadex limits 5000 Daltons
Vitamin B12 : 1350 Daltons

Ve vitamin B12  = 8,7 mL
Vt sephadex G-25 = 5,3 mL
Vo = 5,7

I don't understand why Ve > Vt, is it possible?

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