Western blot vs. immunoprecipitations??

Neal Robert Melvin nrmelvin at ucalgary.ca
Wed Nov 15 03:12:31 EST 2000

What would the likely reason be behind the finding that often a particular
antibody shows a lot of non-specific bands on a western, but only the
ones known to be specific after an IP?? Do antibodies have higher
affinity and specificity for their antigens when the they are allowed to
interact in solution in the IP condition, as opposed to just on a western
blot alone??


"If there are many universes, there will be one where there is a set of
numbers suitable to life... we are in that one"

Martin Rees, Astronomer Royal 

Neal Melvin
University of Calgary - Faculty of Medicine
Department of Neuroscience - Neuroscience Research Group (NRG)
Health Sciences Centre
3330 Hospital Drive, NW
Calgary, Alberta, Canada
T2N 4N1

e-mail: nrmelvin at ucalgary.ca
Phone: (403) 220-4490 (lab)
       (403) 220-7035 (office)
       (403) 283-8731 (fax)

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