Netrophil isolation problem

Alberto Clivio alberto at mailserver.unimi.it
Thu Nov 16 04:30:21 EST 2000


Dear Bryan,

I think there are 2 solutions to your problem:
1) check the molecular weight of your Dextran: it usually works very well with
high molecular weight dextran (>500.000).

2) you might use a special "Mono-poly resolving medium" you can get from
different companies, which avoids the dextran separation step and gives two
sharp bands of white cells: the upper band is mononuclear cells, the lower one
granulocytes. RBC sediment at the bottom of the tube.

Alberto Clivio
Milano, Italy






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