Removal of SDS

Frankie s941874 at mailserv.cuhk.edu.hk
Fri Nov 17 04:00:16 EST 2000


We purified an antibody in SDS-PAGE gel and recovered the antibody from
the gel using electro-elution.  We found that the recovery rate was poor
unless SDS containing elution buffer was used.  However, the presence of
SDS in the elutant is not desirable in the following experiment.  Can
anyone suggest us an effective way to remove the SDS from the elutant or
any effective non-SDS elution buffer for electro-elution.  Thank you.

 TAM Frankie


---






More information about the Immuno mailing list