Neutrophil isolation problem

Alberto Clivio alberto at mailserver.unimi.it
Fri Nov 17 03:48:43 EST 2000


Dear Bryan,

the Mono-Poly resolving medium does not activate neutrophils, it is just
a special solution of Ficoll-Paque with a higer density as compared to
the usual one. It must be used with whole blood (both heparinized and
with Ca2+ chelating agents) and it works as a single step purification
procedure. Good luck

Alberto Clivio






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