paul.fonteneau fonteno at pharma.u-strasbg.fr
Fri Nov 24 10:47:52 EST 2000

Rajabather Krishnaraj wrote:
> If I have a capsule or tablet of an extract from a plant material, (and
> is generally believed to be non-toxic), what would be minimum number and
> type of critical immunological tests I would need to perform (in a mouse
> or rat model) to prove at least initially that the extract is not a
> general immunosuppressant?

For a response considered as preliminary ("initially"...) :
- antibody responses in vivo against soluble antigen (eg ovalbumin) and
particle antigen (eg sheep red blood cells)
- in vitro tests :
	*mixed lymphocyte reaction
	*response to mitogens (eg ConA for T cells, LPS for B cells)
	*Cytotoxic activity of NK cells
- comparison of the number of cells (B cells, T cells CD4 or CD8,
antigen-presenting cells) in spleen of animals treated and untreated

Really preliminary experiments, but giving informations on the state of
the immune system.

(sometimes immunologist)

More information about the Immuno mailing list