Question about Protein A purification of IgG
cpichon at club-internet.fr
Mon Oct 9 16:30:54 EST 2000
aeon a écrit :
> I am a Med.Sci. student who is really desiring the answers to these two
> questions for an upcoming report.
> 1. What is the molecular basis for the selective binding of IgG by protein
It is an affinity chromatography by hydrophobic interaction of antibody Fc and
> 2. What is the difference between Protein A and Protein G in relation to
> their Ig class binding specificities?
Complexe,often the protein G is used to purifie mouse Ig ( low affinity in
comparaison with other species, eg rabbit, horse, human, rat,) and espacially
Ig G1 ( with hight salt buffer ).
> Thank you for your response.
" Mi vida es un erïal:
Flor que toco se deshoja;
Que en mi camino fatal
Alguien va sembrando el mal
Para que yo le recoja. "
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