Question about Protein A purification of IgG

Mark Haynes Mark.Haynes at Mail.TJU.Edu
Tue Oct 10 14:33:19 EST 2000


Hi
THere are probably others with better answers but
protein a is a bacterial cell wall (staph aureas) constituent that binds the
fc portion of immunoglobulin--this helps the bacteria avoid opsinization (i
think) i don't know the exact molecular interactions except i thinks its the
second constant domain on ab that proA binds to.  I think protein g is either
a) a manufactured proA that is either better at binding fc or is more
selective--it may have been discovered in another bacteria but i seem to
recall proG being described as a deletion mutant of proA.

aeon wrote:

> I am a Med.Sci. student who is really desiring the answers to these two
> questions for an upcoming report.
>
> 1. What is the molecular basis for the selective binding of IgG by protein
> A?
> 2. What is the difference between Protein A and Protein G in relation to
> their Ig class binding specificities?
>
> Thank you for your response.
>
> Aeon






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