mAb glycosylation

Mark Haynes Mark.Haynes at Mail.TJU.Edu
Tue Oct 31 12:45:03 EST 2000


I wonder -are phage-made  mab libraries are un-glycosylated?

Tom Barr wrote:

> Hello all,
>     I write requesting advice regarding monoclonal antibody
> glycosylation. I'm aware that different mAbs have different degrees of
> glycosylation, and that the same mAbs, produced under varying conditions
> can display various levels of glycosylation. My question is this, can a
> mAb be non-glycosylated and is their an assay (preferably simple and
> quick!) to determine the presence/quantity of carbohydrate moieties on
> mAbs ? Any advice would be greatly appreciated...
>
> Cheers
>
> Tom
>
> --
> -----------------------------------------------
> Tom Barr (mdp96tab at shef.ac.uk)
> Division of Genomic Medicine (F-Floor)
> University of Sheffield Medical School
> Beech Hill Road
> Sheffield
> S10 2RX






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