Heavy/light chains on Coomaasie gel??

jeff frelinger jfrelin at med.unc.edu
Tue Oct 31 18:03:40 EST 2000


There is twice as much protein mass in a H than than a light chain
"Neal Robert Melvin" <nrmelvin at ucalgary.ca> wrote in message
news:Pine.A41.4.10.10010240215150.86132-100000 at acs4.acs.ucalgary.ca...
> Hello,
>
> I've been using Coomassie Blue staining to get a rough idea of the
> concentration of normal rabbit serum. When I do this, I notice that the
> bands corresponding to the heavy chains stain significantly more intensely
> than the light chain bands... should these be the rough the same intesity
> since one IgG contains 2 heavy chains and two light chains??
>
> I'm trying to match the concentration of this normal rabbit serum to a
> known amount of immune rabbit serum so that I can perform an
> immunoprecipitation with equivalent amounts of IgG's... which bands should
> I try to match in intensity (between my immune serum and my non-immune
> serum), the heavy chains of each, or the light chains??
>
> Neal
>
> "If there are many universes, there will be one where there is a set of
> numbers suitable to life... we are in that one"
>
> Martin Rees, Astronomer Royal
>
> Neal Melvin
> University of Calgary - Faculty of Medicine
> Department of Neuroscience - Neuroscience Research Group (NRG)
> Health Sciences Centre
> 3330 Hospital Drive, NW
> Calgary, Alberta, Canada
> T2N 4N1
>
> e-mail: nrmelvin at ucalgary.ca
> Phone: (403) 220-4490 (lab)
>        (403) 220-7035 (office)
>        (403) 283-8731 (fax)
>
>
>
>
>
>







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