ELISA and standard

Joerg Schenk jschenk at mdc-berlin.de
Fri Apr 6 04:45:28 EST 2001

Hi Saskia,
there is no general answer to this question. It depends on what you standard
is. Usually there is no need to freeze it at -70°C, -20°C should be
There are some proteins that do not bind after freezing, so these should be
kept at 4°C.
Hope this helps


> Can anyone tell me if I would have a problem with my ELISA if I kept my
> standard in the -20° C freeze. We're having a discussion in our lab wether
> to keep our standards at -20° C or -70° C.

Joerg A.Schenk
Max-Delbrueck-Centrum fuer Molekulare Medizin (MDC)
Robert-Roessle-Strasse 10
D-13092 Berlin-Buch, GERMANY
Tel.: +49 (30) 9406 2169
Fax.: +49 (30) 9406 3895
private: +49 (30) 4451540

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