B cell transfection

Toni toni10 at ibv.csic.es
Tue Apr 17 10:24:48 EST 2001


	Hi Anna,
		You must know what is the host range of the retroviruses that you are
making. If your cells are human you need an amphotropic or pantropic
packaging cell line. If are murine you can use  an ecotropic ( it is
more safe, you can infect your self with the pantropic and the
anphotropic viruses).
	Other important thing is the proliferation of the target cells.
Retroviruses only infect proliferating cells.
	Good luck !
	Toni

Anna Lopukhina wrote:
> 
> Hello!
> I am trying to infect pre-B cell v-abl transformed cell line with
> retrovirus supernatent (to get it I transform 293 T cells), but I have
> no infection whatsoever.If anyone could help me with resolving the
> problem I will be very grateful. Thank you very much.
> Anna Lopukhina.
> analopina at yahoo.com




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