Purifying antibody

gs207 at yahoo.com gs207 at yahoo.com
Tue Aug 14 11:16:11 EST 2001


Hi

I am new to the field of Immunology etc, so please excuse me if I ask
something stupid.  I want to use an antibody in immunoprecipitation
assays .  All I have been provided with are bleeds from rabbit immunized
with peptide from the protein of interest.  The peptide has a cysteine
at amino terminus.  I have been told that I need to purify that antibody
with that peptide before using it.  I have no idea how to go about it.
I would really appreciate any help with this.  If you can guide me to a
published protocol (better if you can provide me with one you have used)
and/or any kits I can use.  Thanks a lot.




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