EGFP

Laszlo Radvanyi laszlo55 at earthlink.net
Sat Dec 15 20:57:55 EST 2001


So far as I know there are no defined class I or class II epitopes for GFP.
But, if there is anyone out there that knows of one that would be great.
An interesting suggestion may be to make a fusion of some sort between B-gal
and a short sequence having defined epitopes separated by a short peptide
sequence that can be cleaved by the proteasome.

Laszlo Radvanyi

"Simon Frost" <frostie.football at btinternet.com> wrote in message
news:9tbqpb$fhf$1 at neptunium.btinternet.com...
> Hi,
>  I'm looking for memory responses in T cells educated with Dendritic cells
> transfected with Flu NP as part of my project. I use DC's transfected with
> EGFP expressing  plasmid to monitor transfection efficiency. However, when
I
> measure T cell activation by ELISPOT the EGFP gives a high background in
> some but not all donors. Does anybody know of any characterised epitopes
> within EGFP or which HLA backgrounds are responsible.
>
> thanks in advance
>
>
> frostie
>
>





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