Apoptosis induction and quantification
daniel_blount at hotmail.com
Tue Dec 18 18:22:39 EST 2001
I would like to carry out an adaptation of the apoptosis assessment assay
used by Monti et al. (Mech. Age. Dev. 121: 239-250). Briefly, it would
involve the incubation of whole blood with and without 2-deoxy-D-ribose (as
an oxidative apoptosis inducer) for 48 hours. Apoptosis would then be
measured with Annexin V and PI on the flow cytometer. I cannot find a
protocol on the internet where somebody has carried this out before and so
do not know what quantity of 2-deooxy-D-ribose should be used. Any comments
or advice on this procedure would be most gratefully received.
Daniel G. Blount
School of Pahrmaceutical Sciences
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