Apoptosis induction and quantification

Daniel Blount daniel_blount at hotmail.com
Tue Dec 18 18:22:39 EST 2001


I would like to carry out an adaptation of the apoptosis assessment assay 
used by Monti et al. (Mech. Age. Dev. 121: 239-250). Briefly, it would 
involve the incubation of whole blood with and without 2-deoxy-D-ribose (as 
an oxidative apoptosis inducer) for 48 hours. Apoptosis would then be 
measured with Annexin V and PI on the flow cytometer. I cannot find a 
protocol on the internet where somebody has carried this out before and so 
do not know what quantity of 2-deooxy-D-ribose should be used. Any comments 
or advice on this procedure would be most gratefully received.

Regards

Daniel G. Blount
Nottingham University
School of Pahrmaceutical Sciences
University Park
Nottingham
NG7 2RD
UK


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