Purifying small volumes of antibodies

Nanci E Donacki nancieed at erols.com
Fri Feb 2 16:18:14 EST 2001


Phil,
I have used those before.  The issue with them for purification is that
their capacity is much lower than the gel matrices.

Thanks
-ned

"Phlebas" <phlebas at talk21.com> wrote in message
news:95ess3$bbn$1 at nnrp1.deja.com...
> Have you thought of protein G coated magnetic beads? Dynal sell
> Dynabeads for purification of antibodies from volumes of up to 1 ml, if
> your concentration is > 1 ug/ml (you could do your 2 ml twice?). See
> http://www.dynal.no for details.
>
> Phil
>
> > I am looking for some suggestions to purifiy small volumes (~2 ml
> supe) of
> > monoclonal antibodies.  I've found filter plates from several
> manufacturers
> > for DNA purifications, but not proteins.
> > I am currently looking at using filter plates with small amounts of
> protein
> > G, then eluting and collecting into 96 well plates.  I'm concerned
> that I'll
> > lose more than recover.
> > Any suggestions?
> >
> > Nanci
> >
> >
>
>
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> http://www.deja.com/







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