ELISA + PVDF

Elizabeth Lee elizabeth at jhs.com.sg
Mon Feb 5 22:08:06 EST 2001


I have 2 questions -

1)  ELISA using 96 well plates coated with PVDF menbrane vs the regular
Nunc maxisorb plates, what is the big difference about it? Does PVDF
coated plates give better readings, better adsorption of the antibody?

2)  when using PVDF coated plates, do I have to dilute the capture
antibody in coating buffer or what kind of buffer?

Thanks for your help

Elizabeth


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