MAbs and Western Blot.

Mark Haynes Mark.Haynes at Mail.TJU.Edu
Mon Jan 22 17:50:02 EST 2001

If I could be so bold, what kind of development work, are these developmental
antigens? What are the Mabs directed toward, and what is the immunoprecipitation
Its not uncommon for Mabs to work differently in different tests, especially for
some antigens.

Joe Chandler wrote:

> In the course of our development work, we have generated scores of MAbs.
> They work in beautifully in EIA and IHC.  However, we seem to find that even
> high binding MAbs do not often work well in Westerns.  I have done a
> literature search on this and have not found much to help me analyze this.
> Any thoughts from this illustrious group?

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