"Mark Haynes" <Mark.Haynes at Mail.TJU.Edu> wrote in message
news:3A6CB91A.C4333144 at Mail.TJU.Edu...
> If I could be so bold, what kind of development work, are these
developmental antigens? What are the Mabs directed toward, and what is the
immunoprecipitation data?
Its not uncommon for Mabs to work differently in different tests, especially
for some antigens.
The immunogens supplied to us are varied. Most often, they are peptides or
fusion proteins representative of a cell constituent. I think from what I
have gathered thus far, that the denaturation of the target molecules
changes it sufficiently enough to make the monoclonal binding antibody not
recognize the same site as it did when the molecule was in its native form.
The answer for us is to generate more fusion products in the hopes that we
will find one that will recognize a sequence that is unchanged during the
denaturation process.
Thanks to everyone who send my their comments. Very illuminating.
Joe
