cryopreservers for E. coli O157
H.Ternent at vet.gla.ac.uk
Fri Jan 26 06:09:34 EST 2001
Can anyone be of any assistance.
I am trying to isolate E. coli O157 and due to the sensitivity of the research, I have to store samples for at least 6 months at -80. Samples are pre-enriched in buffered peptone water and I'm looking for information on the best cryopreservative for a 10 ml sample which may or maynot contain E. coli O157 and if it does it may very well be in a stressed state.
Any suggestions of what to use?
(Samples are swabs looking at bovine faecal contamination - related to food products)
I am trying glycerol and have have been advised to try trehalose, but any other information or suggestions would be most welcome.
Mrs Helen E. Ternent
Comparative Epidemiology & Informatics
Department of Veterinary Clinical Studies
University of Glasgow
Email H.Ternent at vet.gla.ac.uk
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