Purifying small volumes of antibodies

Nanci E Donacki nancieed at erols.com
Tue Jan 30 21:04:10 EST 2001


I am looking for some suggestions to purifiy small volumes (~2 ml supe) of
monoclonal antibodies.  I've found filter plates from several manufacturers
for DNA purifications, but not proteins.
I am currently looking at using filter plates with small amounts of protein
G, then eluting and collecting into 96 well plates.  I'm concerned that I'll
lose more than recover.
Any suggestions?

Nanci







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