Purifying small volumes of antibodies

Nanci E Donacki nancieed at erols.com
Tue Jan 30 21:04:10 EST 2001

I am looking for some suggestions to purifiy small volumes (~2 ml supe) of
monoclonal antibodies.  I've found filter plates from several manufacturers
for DNA purifications, but not proteins.
I am currently looking at using filter plates with small amounts of protein
G, then eluting and collecting into 96 well plates.  I'm concerned that I'll
lose more than recover.
Any suggestions?


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