preparation of single cell suspensions
BH711 at hotmail.com
Wed Jan 31 16:42:13 EST 2001
hsnel at nizo.nl wrote in <C12569E3.004EC747.00 at mail.nizo.nl>:
>I am looking for equipement for dissociation of lymphoid organs into
>single cell suspension. What company sells this in Holland/Europe? And
>why do most papers refer to stainless steel sieves? Other (i.e.
>disposable) material cannot be used for this purpose?
We gave up using the stainless steel sieves. We now use the disposable
ones ,no more autoclaving, contamination..etc just throw it away and use a
Becton Dickison/Falcon Sterile 40,70,100 micron mess. Fits right over a
50ml centifuge tube. Break up the spleen/node right into the tube use a
syringe plunger. These are the way to go. There really is no reason not to
use them except "that's the way it was always done"
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