Mark Haynes Mark.Haynes at Mail.TJU.Edu
Mon Jul 2 09:41:36 EST 2001

what do you mean by cell-elisa?
are you staining cells and reading in a flourimeter?
are you doing elispot assays that detect secreted products?
are you assaying a cells ability to perform an enzyme reaction?
these all have been done and they are all different.

yangxx wrote:

> is there anyone doing the cell-elisa?if someone did it ,can you giceme  the
> protocol.and i have   some questions:
>  1)if  A450 of negative control( using the irrelevance cells) is high,how
> can i reduce this.
> 2)if i use the cell-culture plate but not the plate that is special for the
> cell-elisa to do this expriment,is there any  influence to  the results?
> thanks a lot!
> <http://www.biowww.net/forum/read.php?f=3&i=427&t=427>

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