Mark.Haynes at Mail.TJU.Edu
Mon Jul 2 09:41:36 EST 2001
what do you mean by cell-elisa?
are you staining cells and reading in a flourimeter?
are you doing elispot assays that detect secreted products?
are you assaying a cells ability to perform an enzyme reaction?
these all have been done and they are all different.
> is there anyone doing the cell-elisa?if someone did it ,can you giceme the
> protocol.and i have some questions:
> 1)if A450 of negative control( using the irrelevance cells) is high,how
> can i reduce this.
> 2)if i use the cell-culture plate but not the plate that is special for the
> cell-elisa to do this expriment,is there any influence to the results?
> thanks a lot!
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