cell-elisa

William Joseph Kokolus cm006 at bfn.org
Thu Jul 12 21:23:39 EST 2001


One possible reference for cellular ELISA's:

A cell surface ELISA for the screening of monoclonal antibodies to
antigens on viable cells in suspension

J. Immunological Methods 171 (1994) 93-102

Best regards,

William J. Kokolus, Ph. D.
President, Rainbow-Lightning Biomedicine
69 Ferndale Ave.
Kenmore, N.Y., U.S.A., 14217-1003
telephone: 716 873-6940
fax:       716 873-6940

On Mon, 2 Jul 2001, Mark Haynes wrote:

> what do you mean by cell-elisa?
> are you staining cells and reading in a flourimeter?
> are you doing elispot assays that detect secreted products?
> are you assaying a cells ability to perform an enzyme reaction?
> these all have been done and they are all different.
> markH
> 
> yangxx wrote:
> 
> > is there anyone doing the cell-elisa?if someone did it ,can you giceme  the
> > protocol.and i have   some questions:
> >  1)if  A450 of negative control( using the irrelevance cells) is high,how
> > can i reduce this.
> > 2)if i use the cell-culture plate but not the plate that is special for the
> > cell-elisa to do this expriment,is there any  influence to  the results?
> > thanks a lot!
> >
> > <http://www.biowww.net/forum/read.php?f=3&i=427&t=427>
> 
> 

William Joseph Kokolus, Ph. D.
President, Rainbow-Lightning Biomedicine
69 Ferndale Ave.
Kenmore, N.Y., U.S.A., 14217-1003
telephone: 716 873-6940
fax:       716 873-6940
email:     cm006 at bfn.org
           kokolus at att.net

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