TCR transgenic

Jennifer Kraft jkraft at
Fri Jul 13 15:10:27 EST 2001

I was wondering if anyone could give me any advice on some TCR transgenic
mice we had generated.  These mice are transgenic for a Va2, Vb4 TCR that
recognizes a 9-mer peptide (77.2) derived from a T. cruzi protein called
TSA-1 in the context of H-2Kb.  We have Kb-77.2 tetramers which bind a
fairly large percentage of our lymphocyte gate (in both peripheral blood and
spleen), however very few of these cells express CD8 or CD4, and most are
double negative.  So far I have been unable to see any peptide specific
function, either.   Does anyone have any suggestions for backcrossing or any
theories about why these cells are not selecting properly?

Thank you,
Jennifer Kraft

Jennifer R. Kraft, Ph.D.
Department of Cellular Biology
724 Biological Sciences Building
University of Georgia
Athens, GA 30602
(706) 542-3396


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