Mark.Haynes at Mail.TJU.Edu
Mon Jul 16 12:46:31 EST 2001
the answers to the questions depend on how the blood is collected.
w or w/o heparin or other anti-coagulant.
the lyophilization is done with a machine.
hopefully, there is one available to you.
otherwise aliquot and freeze the sera after spinning the coagulated-blood.
Tomasz Czernecki wrote:
> Hi All
> I am research worker in Department of Food Technology, Agricultural
> University in Lublin, Poland. My research work concerns food allergy.
> I have question. How to prepare patient's sera from full blood patients with
> allergy (sera contains specific IgE) and how to do lyophilisation this sera
> I would greatly appreciate receiving a description (step by step) how to do
> sera and lyophilisation. It's my first work with human blood.
> Thanking you for this courtesy.
> Tomasz-Czernecki at wp.pl
> TomaszCz at Agros.ar.lublin.pl
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