Immunoprecipitation

Natalie Sampson nsampson at hgmp.mrc.ac.uk
Tue Jul 24 06:01:31 EST 2001


I am trying (without success) to pull down a transiently expressed c-myc epitope tagged protein.  From immunocytochemistry labelling, my protein is a soluble nuclear factor.  From the precipitation experiments I have done so far, I am getting several background (unwanted proteins) and not mine.  It is definitely present in the transfected cells (western blot analysis on total cell lysates).  Does anyone have any suggestions.  I have tried numerous washing buffers (RIPA, high salt etc) and am fast running o
ut of ideas.  Can anyone  help?

Thank you 

Natalie


nsampson at hgmp.mrc.ac.uk

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