monocyte cell culture

Bryan Heit bjheit at ucalgary.ca
Mon Jul 30 16:48:47 EST 2001


You can culture using RPMI + 20% FCS, although the human serum would be
better.  You can culture on plain plastic, although removing the cells
can be a pain
and may  require trypsanization.

Hope this helps

Bryan Heit
Immunology Research Group
University of Calgary
Calgary, Canada


Eric wrote:

> Has anyone had any experinec in culutring human
> monocytes? Specifically can I use FCS instead on human AB serum to
> maintain the cells.Also must I culture the cells on special (polyprop
> or Teflon coated) flasks?thanks in advance  Eric deszo at uiuc.edu




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