Protease Assay

paul.fonteneau fonteno at
Tue Jun 5 09:05:13 EST 2001

lab508 wrote:
> we want to measure the activity of a recombinant Granzyme-B-construct.
> The assay we have found so far is described in a paper from J.L. Harris
> et al., 1998, Journal of Biol. Chem. - in this assay the substrate was
> prepared in Me2SO - can anybody  tell us, what this solution exactly is
> ?

I suppose that it's DMSO (dimethyl sulfoxide), which is used to
solubilize hydrophobic reagents, in non-toxic conditions.

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