mmanjili at yahoo.com
Thu Mar 15 14:10:05 EST 2001
I have a problem with immunoprecipitation (ip) in that I get non-specific
binding of recombinant proteins to protein A sepharose while using RIPA
buffer or PBS. I incubate antibody-antigen at 4 degree for 2 h and then add
protein A sepharose to the mixture followed by incubation at 4 degree
overnight while rotating.
More information about the Immuno