Immunoprecipitation

Manjili mmanjili at yahoo.com
Thu Mar 15 14:10:05 EST 2001


I have a problem with immunoprecipitation (ip) in that I get non-specific
binding of recombinant proteins to protein A sepharose while using RIPA
buffer or PBS. I incubate antibody-antigen at 4 degree for 2 h and then add
protein A sepharose to the mixture followed by incubation at 4 degree
overnight while rotating.

Thanks,
Manjili


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