dendritic cells purification

[vuvu at freemail.it]

Hi
I have problems in immunomagnetic purification of dendritic cells  from
human buffy coats. I'm doing a positive selection of CD14 cells  and I
get variable purity which ultimately affects consistently the quality of
the cells at later steps. I think that the problem might be at the
column step when I notice clumps formation.  I'd greatly appreciate if
you could give me your advice.
Thanks
Daniela

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