How to remove inviable dead cells in tissue culturing

Paula Lavery Paula.Lavery at
Tue Mar 27 10:15:50 EST 2001

Hi Eddie

You may want to try spinning your cells over a Ficoll-Paque gradient. I've
done this in the past to clean up BLCL cultures. If your cells are not
human, there are other, similar products you can use (check out Cedarlane

Good luck!

Paula Lavery
Transplant Immunology Lab.
Royal Victoria Hospital
McGill University
Montreal, QC, Canada

"If we knew what we were doing, it wouldn't be
called research, would it?" - Albert Einstein

EddieWK <ewkip at> wrote in message
news:99ntse$17343 at
> Hi there,
> I'm currently culturing a particular cell line, that's kind of suspension
> cells.  I would like to know how to remove those inviable dead cells
> a SINGLE passage efficiently.  I'm actually quite new at this area.
> suggests. Thanks.
> Eddie

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